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  熒光光纖氧氣測量技術具有高精確度、高可靠性、響應時間短、適用于氣相和液相等優(yōu)勢，因此隨著技術的問世，精確、高通量測量微小生物的呼吸和評估其能量代謝成為可能。高通量呼吸測量系統(tǒng)基于熒光光纖氧氣測量技術，能夠?qū)?strong>斑馬魚的胚胎及幼魚進行測量，測定其耗氧量，進而評估其代謝水平。系統(tǒng)在生物醫(yī)學、實驗生物學、污染生態(tài)學與環(huán)境毒理學、環(huán)境科學、氣候變化研究等領域具有越來越重要的應用價值。

左：斑馬魚微型呼吸室；右：斑馬魚高通量呼吸代謝測量系統(tǒng)測量原理

  系統(tǒng)由內(nèi)置熒光光纖氧氣傳感器的微型呼吸室、氧氣測量主機及數(shù)據(jù)采集分析軟件組成，可對96個通道的樣品進行同步測量。

功能特點

• 氧氣測量高精度、高可靠性、低功耗、低交叉敏感性、快速響應時間
• 輕松校準
• 非侵入性和非破壞性測量
• 緊湊設計，適用于溫控培養(yǎng)箱和/或搖床

技術參數(shù)

1. 檢測技術：光纖氧傳感器技術。
2. 適用場景：原位檢測，可在培養(yǎng)箱里或搖床上使用，便于溫度控制。
3. 呼吸室：透明聚苯乙烯材質(zhì)，支持預消毒處理，可重復使用。
4. 氧氣測量主機：單個重670 g，162 x 102 x 32 mm
5. 主機內(nèi)置溫度傳感器：0-50°C，分辨率012°C，精度±0.5°C
6. 主機內(nèi)置壓強傳感器：300-1100mbar，分辨率11mbar，精度±6mbar
7. 最大采樣頻率：單通道激活時可達10-20次每秒
8. 氧氣測量精度：±0.1% O2@1% O2或±0.05 mg/L@0.44 mg/L
9. 氧氣測量分辨率：01% O2@1% O2或0.005 mg/L@0.44 mg/L
10. 電源：5VDC，USB供電
11. 響應時間＜30s
12. 通道數(shù)：96
13. 系統(tǒng)適配其他魚類的胚胎及幼魚
14. 可選配斑馬魚成魚的靜態(tài)及動態(tài)呼吸測量系統(tǒng)

左圖：環(huán)境污染物6PPD和6PPD醌對斑馬魚幼魚呼吸的影響(Varshney et al., 2022)；

右圖：小丑魚幼魚在不同溫度下的代謝率(Moore et al., 2023)

參考文獻

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